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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Biofabrication. 2012 Apr 23;4(2):025004. doi: 10.1088/1758-5082/4/2/025004

Figure 9 . 9B, 9C. Adipogenic, and osteogenic differentiation of ASC spheroids in suspension culture.

Figure 9

Figure 9

Human ASC spheroids were cultured for 2 weeks in serum-free suspension (LGFM-SF) and then exposed to either adipogenic or osteogenic medium for 3 weeks. Control spheroids remained in LGFM-SF medium for a total of 5 weeks. Oil Red O staining of spheroid sections grown in control (left upper) or adipogenic (right upper) medium demonstrates notable adipogenesis in the latter. Alizarin Red staining of spheroid sections grown in osteogenic medium (right lower) demonstrates the presence of calcified matrix. Figure 9B shows control spheroids in LGFM-SF medium and Figure 9C demonstrates Alcian Blue staining of spheroid section grown in osteogenic medium.