Fig. 2.
Experimental validation of pipette-based LFP. All sample fluids were added with a minimum of 3 seconds between each event. A. Simple three step description of the loading protocol. B. Three frames picturing a channel with equal ratio patterning at different time-points of the process. C. Image at the end of the flow in a channel designed to split the culture channel into 1/3 and 2/3 sections. D. Fluorescent and bright field merged images of Alexa488 dye flow in the 1/2-1/2 patterning device (left) amd the 1/3-2/3 device (right) and a the respective cross section fluorescent intensity profile.
