Fig. 4.
A. Demonstration of a wound-healing/migration assay with variable spacing between the two cell populations. The upper right diagram represents the microfluidic device geometry. B. Multiple contrast enhanced movie-frames superimposed to illustrate the flow paths of cells being patterned in the wound-healing/migration device. C. RFP and GFP MCF7 cells patterned into a three-flow device. D. Osteoblasts (MC3T3-E1) and prostate cancer cells (LNCaP) are patterned within in a collagen matrix. The inset shows collagen fibers formed after polymerization using phase contrast imaging. E. Illustration of the arraying ability of the method for high-throughput screening (HTS) applications. All images were taken within 15 min of seeding.