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. Author manuscript; available in PMC: 2012 Jul 23.
Published in final edited form as: Anal Biochem. 2008 Aug 30;383(1):18–24. doi: 10.1016/j.ab.2008.08.023

Table 4.

Intraassay variation and recovery for HepG2 cells.a,b,c,d

Compound Input (nmol) Mean (nmol) CV (%) Recoveryd (%)
MVA 1.00 0.98 9.8 98
MVA-P 1.00 1.14 9.9 114
MVA-PP 1.00 1.06 10.3 106
IPP 0.20 0.22 10.3 109
GPP 0.10 0.12 4.3 124
FPP 0.11 0.13 6.0 116
GGPP 0.12 0.13 5.8 106
Compound Input (nmol) Mean (nmol) CV (%) Recoveryd (%)
MVA 1.50 1.48 9.6 99
MVA-P 1.50 1.63 7.1 108
MVA-PP 1.50 1.60 9.6 106
IPP 1.50 1.48 8.4 98
GPP 1.50 1.44 4.7 96
FPP 1.50 1.61 5.6 108
GGPP 1.50 1.62 4.1 108
Compound Input (nmol) Mean (nmol) CV (%) Recoveryd (%)
MVA 3.00 2.96 10.9 99
MVA-P 3.00 2.94 7.8 98
MVA-PP 3.00 2.90 8.7 97
IPP 3.00 2.74 8.8 91
GPP 3.00 2.90 3.7 97
FPP 3.00 3.11 4.3 104
GGPP 3.00 3.08 3.6 103
a

n = 10 for each compound concentration.

b

No intermediates were detected in HepG2 cell homogenates without supplementation.

c

Every sample contains 775 μg of protein.

d

Recoveries were determined using cell homogenates supplemented with the indicated intermediates.