Table 5.

Interassay variation and recovery for HepG2 cells.^a,^b,^c,^d

Compound	Input (nmol)	Mean (nmol)	CV (%)	Recoveryd (%)
MVA	1.00	1.02	9.5	102
MVA-P	1.00	0.99	10.2	99
MVA-PP	1.00	1.06	11.9	106
IPP	0.20	0.22	11.2	109
GPP	0.10	0.12	8.5	123
FPP	0.11	0.12	11.1	107
GGPP	0.12	0.11	11.1	94
Compound	Input (nmol)	Mean (nmol)	CV (%)	Recoveryd (%)
MVA	1.50	1.62	10.6	108
MVA-P	1.50	1.52	9.5	101
MVA-PP	1.50	1.47	11.5	98
IPP	1.50	1.54	10.6	103
GPP	1.50	1.61	9.6	108
FPP	1.50	1.69	9.3	113
GGPP	1.50	1.61	4.6	107
Compound	Input (nmol)	Mean (nmol)	CV (%)	Recoveryd (%)
MVA	3.00	3.12	8.5	104
MVA-P	3.00	2.88	9.8	96
MVA-PP	3.00	2.81	10.5	94
IPP	3.00	2.82	9.9	94
GPP	3.00	3.05	4.4	102
FPP	3.00	3.14	6.7	105
GGPP	3.00	3.09	7.7	103

^an = 10 for each compound concentration.

^bNo intermediates were detected in HepG2 cell homogenates without supplementation.

^cEvery sample contains 775 μg of protein.

^dRecoveries were determined using cell homogenates supplemented with the indicated intermediates.