Figure 4. Glycans at positions 234 and 295 increase HIV-1 sensitivity to GRFT, CV-N and SVN.
Glycosylation sites at 234 and/or 295 were introduced in CAP206.08J, CAP63.A9J and CAAN5342.A2 by site-directed mutagenesis. Mutant viruses were tested in neutralization assays against GRFT (A), CV-N (B) and SVN (C) in JC53bl-13 cells. Each virus was tested at least three times.