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. 2012 Apr-Jun;2(2):241–249. doi: 10.4103/2045-8932.97616

Figure 2.

Figure 2

(A) Immunoblot of HIF-1α and HIF-1β (loading control) in PASMCnorm. Cells were treated for 4 hours in either hypoxic medium (Hyp) or normoxic medium (Norm) with or without DMOG at concentrations of 10 μM, 100 μM and 1 mM. Hypoxia and DMOG at the two higher concentrations stabilized HIF-1α and the DMOG dose of 100 μM was chosen as being closest to the physiological level of hypoxia. Both hypoxia and DMOG (100 μM) inhibited [3H]-Thymidine incorporation in PASMCnorm (B) and increased [3H]-Thymidine incorporation in PASMChyp (C). n=3; *P<0.05 vs. normoxia.