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Melting temperatures (Tm) for PNA:DNA and PNA:RNA duplexes, measured by UV absorbance at 260 nm in PBS buffer at pH 7, in the absence or in the presence of urea (5 m). Strand concentration: 5 μm.
| PNA[a] | DNA1 in water | DNA1 in urea (5 m) | DNA2 in urea (5 m) | RNA1 in water | RNA1 in urea (5 m) | RNA2 in urea (5 m) |
|---|---|---|---|---|---|---|
| PNA1 | 85 | 70 | 58 | 88 | 77 | 62 |
| PNA2 | >90 | 80 | 68 | >90 | 88 | 73 |
| PNA3 | >90 | 77 | 65 | >90 | 76 | 60 (br) |
| PNA4 | >90 | 77 | 56 | >90 | 86 | 75 |
| PNA5 | >90 | 74 | 64 | >90 | 78 | 62 |
| PNA6 | >90 | 77 | 69 | >90 | 75 | 65 |
