Figure 3.

MCA205 cells were injected subcutaneously in C57BL/6 mice and allowed to grow to 5–7 mm in diameter (approximately day 10), whereupon mice were injected intraperitoneally with 250 μg αOX40 or control RatIg and 7 days later the tumour was removed. (a) CD11b⁺ tumour macrophages from (i) RatIg and (ii) αOX40-treated mice were (b) sub-gated to select only the Gr1^lo IA^hi cells to > 99% purity. Total RNA was isolated from FACSorted CD11b⁺ Gr1^lo IA^hi macrophages and microarray assays were performed in the Affymetrix Microarray Core of the OHSU Gene Microarray Shared Resource. (c) Relative levels of gene expression were determined using GeneSifter. Where multiple probes were matched to an individual gene, representative expression results are shown.