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. 2012 Aug;136(4):448–458. doi: 10.1111/j.1365-2567.2012.03601.x

Figure 6.

Figure 6

Induction of macrophage inflammatory protein-2 (MIP-2) in peritoneal exudate macrophages (PEMs) incubated with ATP requires activation of the extracellular signal-regulated kinase 1/2 (ERK1/2) and p38 mitogen-activated protein kinase (MAPK) signalling pathways. (a) Peritoneal exudate cells (PECs) were incubated with 300 μm ATP at different times. ERK1/2 and p38 MAPK phosphorylation were measured by flow cytometry. ATP-stimulated PECs were stained with anti-phospho-ERK1/2 (pT202/pY204) or p38 MAPK monoclonal antibody (pT180/pY182) (shaded histograms). Unstimulated PECs, which were incubated for 30 min, were stained with isotype control (white histograms). ERK1/2 and p38 MAPK phosphorylation levels were gated on the macrophage population (Mac-1+ cells). One result from three representative experiments is shown. (b) PEMs were pre-incubated for 60 min with an ERK1/2 inhibitor (PD98059) or for 30 min with a p38 MAPK inhibitor (SB202190) before the addition of 300 μm ATP. Stimulation in the presence of these inhibitors continued for 24 hr. Data represent the mean ± SEM of triplicate samples. The experiments were repeated three times.