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. 2011 Jan;3(1):5–20. doi: 10.1002/emmm.201000105

Figure 4. Urinary Hsp72 mRNA and protein levels from rats subjected to different periods of ischemia.

Figure 4

  1. Total RNA was individually extracted from the urine of six rats per group and Hsp72 mRNA levels were determined by real time RT-PCR.
  2. Relationship between mRNA levels and tubular affected area.
  3. Urinary Hsp72 levels assessed by ELISA.
  4. Relationship between urinary Hsp72 levels and the % of tubular affected area.
  5. Urinary Hsp72 levels assessed by WB analysis from four rats of each group.
  6. Relationship between urinary Hsp72 levels detected by WB and tubular injured area. *p < 0.05 versus sham-operated rats, ¤p < 0.05 versus 10 min, €p < 0.05 versus 20 min, £p < 0.05 versus 30 min and ¥p < 0.05 versus 45 min of ischemia group.