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. Author manuscript; available in PMC: 2012 Jul 23.
Published in final edited form as: J Immunother. 2009 Oct;32(8):817–825. doi: 10.1097/CJI.0b013e3181ad4d5d

Figure 5. Efficacy of Affitoxin measured by inhibition of protein synthesis (A) and intracellular ATP level (B).

Figure 5

Five different breast cancer cells lines were transfected with pCMV-GLuc plasmid containing secreted Gaussia luciferase gene under control of strong CMV promoter. Gaussia luciferase activity was measured in the medium 24 hours after treatment with Affitoxin. The same cell lines were assessed for intracellular ATP level 72 hours following treatment (CellTiter-Glo, Promega).