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. Author manuscript; available in PMC: 2012 Oct 21.
Published in final edited form as: ACS Chem Biol. 2011 Aug 18;6(10):1107–1116. doi: 10.1021/cb200168y

Figure 2. proTeOn and proTeOff systems’ design.

Figure 2

a. proTeOn and proTeOff synthetic promoter sequence. Both PROTEON and PROTEOFF bind and recruit RNApol to this synthetic promoter sequence. Moving from 5′ to 3′: the rTetR/TetR protein domain binds tetO, RNApol binds the UP element and the −10 region, LuxRΔN binds the luxbox, the mRNA stability sequence stabilizes the mRNA transcript, and ribosomes bind the RBS of this resulting mRNA message.

b. proTeOn molecular model. Both proTeOn and proTeOff are designed to assemble as shown. The inducible DNA binding domain (rTetR/TetR, blue) binds the tetO operator (purple), and the transcription activator domain (LuxRΔN, orange) binds the luxbox (red). The two domains bind their operators along the same face of the DNA double helix and are connected (TetR/rTetR’s C-terminus to LuxRΔN’s N-terminus) by a linker peptide (green).