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. Author manuscript; available in PMC: 2012 Jul 23.
Published in final edited form as: Exp Hematol. 2011 Feb 4;39(5):570–579. doi: 10.1016/j.exphem.2011.01.014

Figure 5. Characterization of B cells generated from CB HPCs co-cultured with TEC-84.

Figure 5

CB HPCs (2 wells of 2×103) were cultured with TEC-84 for two weeks and analyzed by flow cytometry for the immature B cell subset markers CD79a (PE), CD7 (PECy5), CD19 (PECy7) and intracellular IgM (APC). (A) CD79anegCD7neg cells were not considered to be committed to the B-cell lineage and were negative for two other B cell markers, CD19 and cytoplasmic IgM (cyto µ) (bottom left). CD7negCD79apos cells were considered committed to the B-cell lineage and were classified as preproB, proB, or preB cells based on the expression of CD19 and cyto µ (bottom right). The axes show log10 fluorescence. (B) The total number of B cells represents all cells that were positive for the B cell lineage marker, CD79a. The number of each B cell progenitor was calculated by multiplying the percentage of cells with each B cell progenitor phenotype by the total number of CD79apos cells. The bars indicate the mean ±S.D. of five independent CB HPC cultures.