Table 1.
List of sampling sites sorted by catchment area.
| Sampling site | Code | n | MNA | HE | HO | M ratio | Latitude, Longitude | |
|---|---|---|---|---|---|---|---|---|
| Ems catchment area: | ||||||||
| Category 1. Running/stagnant waters: | ||||||||
| Meckelbach (ru) | MB | 20 | 6.0 | 0.62 | 0.61 | 0.61 ± 0.28 | 51°55′55.66″N,7°34′36.42″E | |
| Mecklenbeck, small pond (st) | SP° | 28 | 5.7 | 0.62 | 0.61* | 0.59 ± 0.29 | 51°56′00.15″N,7°34′33.39″E | |
| Mecklenbeck, large pond (st) | LP | 36 | 5.7 | 0.61 | 0.63* | 0.54 ± 0.25 | 51°55′59.81″N,7°34′30.28″E | |
| Münstersche Aa (ru) | MA | 19 | 6.2 | 0.69 | 0.66 | 0.53 ± 0.33 | 51°56′18.49″N,7°35′08.57″E | |
| Aasee (st) | AS | 21 | 5.7 | 0.66 | 0.63* | 0.57 ± 0.28 | 51°57′21.69″N,7°36′50.34″E | |
| Eschuss Bach (ru) | EB | 23 | 5.3 | 0.62 | 0.59 | 0.59 ± 0.29 | 52°03′20.98″N,7°30′31.40″E | |
| Rain storage reservoir (st) | RSR | 24 | 6.2 | 0.64 | 0.62* | 0.64 ± 0.24 | 52°02′58.76″N,7°29′23.51″E | |
| Hohnebach (ru) | HB | 18 | 4.8 | 0.62 | 0.63 | 0.57 ± 0.34 | 51°56′44.76″N,7°41′13.00″E | |
| Moat Haus Lütkenbeck (st) | MHL | 22 | 4.9 | 0.55 | 0.56 | 0.60 ± 0.34 | 51°56′34.98″N,7°39′35.69″E | |
| Werse (ru) | WE | 22 | 7.1 | 0.67 | 0.67 | 0.56 ± 0.22 | 51°57′47.35″N,7°42′15.30″E | |
| Rieselfelder (st) | RF | 22 | 4.0 | 0.56 | 0.60 | 0.47 ± 0.29 | 52°01′35.96″N,7°39′23.24″E | |
| Category 2. Running waters: | ||||||||
| Loddenbach (ru) | LB° | 20 | 3.3 | 0.37 | 0.36 | 0.49 ± 0.36 | 51°56′10.44″N,7°40′51.83″E | |
| Nienberger Bach (ru) | NB | 31 | 6.3 | 0.68 | 0.71 | 0.56 ± 0.24 | 52°00′15.56″N,7°35′40.66″E | |
| Kinderbach (ru) | KB° | 25 | 7.2 | 0.71 | 0.65 | 0.67 ± 0.24 | 52°00′08.17″N,7°36′48.87″E | |
| Category 3. Salt/freshwater: | ||||||||
| Ibb. Aa, upstream freshwater tributary (f) | IUF | 21 | 5.7 | 0.67 | 0.68* | 0.51 ± 0.24 | 52°15′38.28″N,7°44′24.72″E | |
| Ibb. Aa, freshwater tributary (f) | IF° | 15 | 4.7 | 0.57 | 0.51* | 0.56 ± 0.35 | 52°15′40.99″N,7°44′26.65″E | |
| Ibb. Aa, upstream Aasee (f) | IUA° | 22 | 5.8 | 0.59 | 0.59 | 0.52 ± 0.23 | 52°15′40.09″N,7°44′22.37″E | |
| Ibb. Aa, downstream freshwater tributary (f) | IDF | 20 | 5.2 | 0.62 | 0.62 | 0.53 ± 0.32 | 52°15′41.25″N,7°44′20.82″E | |
| Ibb. Aa, upstream saltwater discharge (f) | 2009 | IUS09° | 23 | 5.3 | 0.62 | 0.57* | 0,49 0,27 | 52°17′00.10″N,7°39′12.76″E |
| 05/2010 | IUS10a | 18 | 4.8 | 0.56 | 0.59 | 0,52 0,32 | ||
| 09/2010 | IUS10b | 18 | 4.9 | 0.58 | 0.60 | 0,50 0,31 | ||
| Saltwater discharge tributary (f) | SDT° | 19 | 3.1 | 0.48 | 0.48 | 0.46 ± 0.32 | 52°17′16.89″N,7°39′05.20″E | |
| Ibb. Aa, directly after saltwater discharge (s) | IDS | 20 | 5.1 | 0.60 | 0.63 | 0.46 ± 0.28 | 52°17′01.03″N,7°39′07.66″E | |
| Ibb. Aa, bridge at Püsselsbüren (s) | IP | 20 | 5.3 | 0.61 | 0.60 | 0.50 ± 0.27 | 52°17′22.16″N,7°38′01.81″E | |
| Ibb. Aa, upstream of Klosterbach (s) | 2009 | IUK09 | 23 | 5.1 | 0.60 | 0.61 | 0,51 0,30 | 52°17′32.47″N,7°36′52.90″E |
| 2010 | IUK10 | 19 | 5.3 | 0.61 | 0.63* | 0,58 0,34 | ||
| Klosterbach (s) | 2009 | KLB09° | 27 | 5.4 | 0.54 | 0.52 | 0,47 0,24 | 52°17′26.28″N,7°36′49.96″E |
| 2010 | KLB10° | 19 | 5.0 | 0.59 | 0.51* | 0,50 0,26 | ||
| Ibb. Aa, downstream of Klosterbach (s) | 05/2009 | IDK09a | 21 | 5.1 | 0.58 | 0.56 | 0,52 0,29 | 52°17′31.76″N,7°36′46.49″E |
| 09/2009 | IDK09b | 21 | 4.7 | 0.56 | 0.61 | 0,59 0,34 | ||
| 05/2010 | IDK10a | 20 | 4.8 | 0.59 | 0.68 | 0,56 0,34 | ||
| 09/2010 | IDK10b | 20 | 5.4 | 0.64 | 0.64 | 0,51 0,24 | ||
| Ibb. Aa, after Midland Channel culvert (s) | IDM | 17 | 5.4 | 0.62 | 0.63* | 0.44 ± 0.18 | 52°17′40.97″N,7°36′27.68″E | |
| Bever, upstream of Salzbach (f) | BUS | 15 | 5.8 | 0.63 | 0.63 | 0.52 ± 0.21 | 52°04′13.16″N,8°03′44.33″E | |
| Salzbach (s) | SB | 20 | 5.0 | 0.59 | 0.66 | 0.54 ± 0.28 | 52°04′12.55″N,8°03′20.45″E | |
| Bever, downstream Salzbach (s) | BDS | 22 | 5.1 | 0.59 | 0.60 | 0.52 ± 0.22 | 52°04′09.03″N,8°03′17.95″E | |
| Weser catchment area: | ||||||||
| Fuhse (f) | FU | 21 | 4.6 | 0.53 | 0.54 | 0.45 ± 0.25 | 52°07′21.91″N,10°22′21.93″E | |
| Mühlgraben (s) | MG° | 22 | 3.1 | 0.41 | 0.46 | 0.54 ± 0.29 | 52°07′05.05″N,10°22′05.72″E | |
| Rhine catchment area: | ||||||||
| Fossa Eugenia, upstream Große Goorley (f) | FUG | 20 | 7.4 | 0.72 | 0.74 | 0.48 ± 0.13 | 52°30′10.70″N,6°31′17.48″E | |
| Große Goorley (s) | GG | 20 | 7.4 | 0.74 | 0.77 | 0.48 ± 0.13 | 51°30′11.99″N,6°31′30.31″E | |
| Mühlenbach (f) | MUB | 21 | 7.8 | 0.73 | 0.77 | 0.52 ± 0.22 | 51°47′13.05″N,7°15′24.49″E | |
| Outgroups: | ||||||||
| Grosser Plöner See, Northern Germany (st) (f) | GPS | 23 | 5.4 | 0.69 | 0.68 | 54°8′38.36″N,10°24′53.23″E | ||
| Limia River, Spain (ru) (f) | SPA° | 20 | 10.0 | 0.67 | 0.63* | 42°8′1.27″N,7°39′47.801″W | ||
| Average | 21.3 | 5.5 | 0.61 | 0.61 | 0.53 | |||
| (standard deviation) | (3.8) | (1.2) | (0.07) | (0.08) | (0.27) | |||
st, stagnant; ru, running water; f, freshwater salinity (approximately 1 mS cm–1), (s) saltwater (> 4 mS cm–1); n, number of fish; MNA, mean number of alleles, HE, expected heterozygosity; HO, observed heterozygosity; Ibb. Aa, Ibbenbürener Aa.
°Population with null alleles (see also Appendix, Table A3).
*
Samples that deviate from Hardy–Weinberg equilibrium (HWE).