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. 2012 Jun;2(6):1251–1259. doi: 10.1002/ece3.198

Figure 2.

Figure 2

Schematic representation of the experimental design. Ten isolates for control (pH 8.0, 20°C) and 10 for selective conditions (pH 7.5, 25°C) of each Alexandrium minutum strain (AL1V and AL2V) were maintained through serial transfer into fresh medium. Under selective conditions, growth rate and toxin cell quota were determined every six months till these traits did not change in three consecutive measurements (720 days, corresponding to ca. 250 generations in strain AL1V and ca. 180 generations in strain AL2V). In order to disentangle the effects of acclimation from genetic adaptation, additional growth rate measurements were made in the controls after ca. 220 generations in strain AL1V and ca. 165 generations in strain AL2V. For this purpose, the control cultures were transferred to selective conditions (pH 7.5, 25°C) for 20 days (corresponding to ca. eight generations in strain AL1V and ca. six generations in strain AL2V).