The induction of BRM-dependent genes. To show that BRM is functional when induced by either RH or GK, the induction of BRM-dependent genes was measured and demonstrated by quantitative PCR in the C33A and SW13 cell lines, which were treated first with either RH 50 μM (a, b) or GK 200 μM (c, d) for 72 h. The DDX58, P8 LGAL and/or XAF1 genes were significantly induced in the C33A (a, c) and SW13 (b, d) cell lines, although these genes were not significantly induced in the anti-BRM shRNAi-derived C33A and SW13 daughter cell lines. The standard error shown represents six experiments for each bar on the graph.