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. Author manuscript; available in PMC: 2013 Jul 1.
Published in final edited form as: Evol Dev. 2012 Jul;14(4):317–325. doi: 10.1111/j.1525-142X.2012.00550.x

Figure 4. Total mRNA expression of tan and ebony differs over developmental time and between species.

Figure 4

The log2 of mRNA abundance at tan (A) and ebony (B) are shown for developmental stages P12 - A1 (C). Each point represents the mean of four log2-transformed biological replicates of either D. americana (blue) or D. novamexicana (red) mRNA expression, relative to D. americana stage A1. D.americana stage A1, the internal reference point, was arbitrarily assigned a log2 value of zero for both genes. Error bars are the experimentwise 95% confidence intervals, calculated separately for tan and ebony.