Figure 5. Species- and allele-specific differences in tan and ebony mRNA expression over developmental time.

The log₂ of mRNA abundance at tan (A) and ebony (B) are shown for developmental stages P12 - A1 (x-axis). Filled circles represent the ratio of D. americana to D. novamexicana gene expression between the species (“parental data”). Open circles represent the ratio of expression of the two alleles within F₁ hybrids. A value of 0 corresponds to no expression difference between the two alleles; alleles are significantly differentially expressed (P < 0.05) at all except stages P12 and A1 (both parental and F₁ data) for tan, and stage P13 (parental data only) for ebony. Parental data were obtained using indirect comparisons for stages P12 and P13, and direct comparisons for stages P14 - A1. Each point shows the mean of four biological replicates. Error bars are experiment-wide 95% confidence intervals, calculated separately for each dataset (parental indirect; parental direct; and F₁ hybrid).