Figure 4.
Nuclear but reduced expression of CMASnls does not affect the general sialylation in Cmasnls kidney homogenates. (A) CMAS staining with S59 in P0.5 kidney sections. Mature glomeruli (black arrows) of the inner cortex are marked. Both CMAS and Cmasnls are localized in the nuclear compartment at single cell level. (B) Immunostaining of CMAS with S56 in cytosolic (c) and nuclear (n) fractions of kidney homogenates at P1.5. Actin expression as internal standard. (C) Total and protein-bound Sia were quantified in kidney homogenates of P1.5 mice by the DMB-HPLC method. Values represent means ± SD of six animals for each genotype. N-acetylneuraminic acid (Neu5Ac, black bars) and N-glycolylneuraminic acid (Neu5Gc, gray bars) were separately assessed.