Figure 5.

Spatial reduction of sialylation in nls/nls glomeruli. (A) Staining of terminal Sia with LFA in P1.5 kidney sections before (−) and after neuraminidase (Neu) treatment. To visualize even weak staining, the tissues were not counterstained. Neu-treated sections are devoid of Sia and serve as negative controls for the LFA staining. Lower lanes represent a larger magnification of the inner rim of the cortex. (B) Staining of terminal galactosyl-β-1,3-N-acetylgalactosamine with PNA in P1.5 kidney sections. Sialylation inhibits PNA binding, whereas Neu treatment uncovers the recognition structure.