Table 1.
Quantification of sialic acid and CMP-sialic acid in kidney of wt and mutant mice
| Nuclear (Cytosolic) Concentration in Kidney | ||||||
|---|---|---|---|---|---|---|
| Sia (nmol/mg protein) | Neu5Ac (%) | Neu5Gc (%) | CMP-Sia (nmol/mg protein) | CMP-Neu5Ac (%) | CMP-Neu5Gc (%) | |
| +/+ | 1.99±0.27 (2.51±0.44) | 76 (74) | 24 (26) | 6.66±0.65 (6.44±0.37) | 70 (58) | 30 (42) |
| nls/+ | 2.76±0.42 (2.11±0.31) | 80 (73) | 20 (27) | 7.44±0.46 (7.07±0.83) | 65 (59) | 35 (41) |
| nls/nls | 3.20±0.94 (2.71±0.83) | 68 (70) | 32 (30) | 6.92±0.80 (7.97±1.32) | 65 (61) | 35 (39) |
Nuclear and cytosolic tissue fractions were prepared from P1.5 kidney. Samples equivalent to 25 µg protein were dried, hydrolyzed, DMB labeled, and separated by HPLC. For the quantification of CMP-Sia, the keto group of free nonactivated Sia was reduced before hydrolysis to prevent DMB labeling. Concentrations of Sia and CMP-Sia were quantified and the percentages of Neu5Ac and Neu5Gc as well as of the activated sugars CMP-Neu5Ac and CMP-Neu5Gc were specified. Cytosolic values are shown in parentheses. Values represent means ± SD of three animals for each genotype.