Figure 2.

tPA-activated NF-κB signaling is receptor annexin A2-mediated and independent of its protease activity. (A) Nonenzymatic tPA (NE-tPA) induced phosphorylation of p65 in a dose-dependent manner. J774 cells were incubated with NE-tPA of indicated concentrations for 6 hours. (B) Knockdown of LRP-1 by RNA silence did not affect tPA-induced NF-κB activation in J774 macrophages. (C) Western blot analysis showed that knockdown of annexin A2 in J774 macrophages abolished tPA-induced NF-κB activation. (D) Quantitative presentation of the relative abundance of phospho-p65 in macrophages treated with control (consiRNA) and annexin A2 (AnxsiRNA) siRNAs. **P<0.01 (n=3). J774 macrophages were incubated with vehicle or 10 nM tPA for 6 hours unless indicated elsewhere.