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. 2012 Aug;23(8):1303–1308. doi: 10.1681/ASN.2011111130

Figure 1.

Figure 1.

IL-2/IL-2Ab complex induces Foxp3+ Treg expansion in vivo. Mice received IL-2/IL-2Ab complex or saline intraperitoneally daily for 3 days. CD4+CD25+Foxp3+ Tregs from splenocytes were stained and analyzed by flow cytometry at day 6. CD4+ Foxp3+ cells increased from 14% to 47%. CD4+CD25+Foxp3+ T cells increased from 9% to 46% (A). CD4+ T cell expansion was greater (57% increased to 64%) than CD8+ T cells (42% decreased to 34%) and NK cells (54% decreased to 14%) (B and D). NK cells were less activated with higher CD62L expression, whereas there was a slight increase in activated CD8+ T cells (CD62L lo) (C). There was an increase in spleen size (E) and increased numbers of Foxp3+ T cells in spleen. (F) Immunohistochemical staining 5 days after IL-2/IL-2Ab complex treatment. Data are representative of three independent experiments (n=5–7). Original magnification, ×400.