Table 1.
Morphometric variables measured in wild-type, heterozygous, and homozygous AA-4E-BP1 Fischer 344 rats with and without prior nephrectomy
| Variable | Wild Type | Heterozygous | Homozygous | |||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Wk 0, 100 g (n=5) | No NX | NX | Wk 0, 100 g (n=10) | No NX | NX | Wk 0, 100 g (n=5) | No NX | NX | ||||||||||||||
| Wk 14 (n=5) | Fold Change over Wk 0 | Wk 14 (n=5) | Fold Change over Wk 0 | Wk 14 (n=5) | Fold Change over Wk 0 | Wk 14 (n=10) | Fold Change over Wk 0 | Wk 14 (n=5) | Fold Change over Wk 0 | Wk 9 (n=5) | Fold Change over Wk 0 |
|||||||||||
| Glomerular tuft volume (μm3) | ||||||||||||||||||||||
| mean | 469,012 | 1,040,081 | 2.2 | 1,394,319 | 3.0 | 465,429 | 1,049,942 | 2.3 | 2,220,893 | 4.8 | 332,473 | 1,285,271 | 3.9 | 2,688,464 | 8.1 | |||||||
| SD | 84,100 | 54,891 | 189,865 | 92,952 | 212,906 | 729,859 | 54,000 | 295,202 | 303,846 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | <0.01 | <0.01 | <0.01 | 0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | 0.03 | 0.01 | NS | NS | 0.02 | NS | <0.01 | |||||||||||||
| GLPP1-positive tuft area (%) | ||||||||||||||||||||||
| mean | 47 | 40 | 0.9 | 36 | 0.8 | 44 | 41 | 0.9 | 22 | 0.5 | 35 | 36 | 1.1 | 6 | 0.3 | |||||||
| SD | 6 | 4 | 4 | 6 | 6 | 18 | 2 | 6 | 3 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | 0.02 | NS | <0.01 | NS | <0.01 | <0.01 | NS | <0.01 | <0.01 | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | NS | <0.01 | NS | NS | <0.01 | NS | <0.01 | |||||||||||||
| GLPP1-positive volume (μm3) | ||||||||||||||||||||||
| mean | 221,747 | 415,217 | 1.9 | 495,051 | 2.2 | 203,676 | 426,971 | 2.1 | 399,118 | 2.0 | 117,259 | 468,379 | 3.9 | 158,365 | 1.4 | |||||||
| SD | 61,861 | 47,401 | 58,717 | 42,568 | 53,570 | 270,773 | 19,676 | 128.703 | 89,343 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | <0.01 | <0.01 | <0.01 | 0.01 | 0.02 | 0.02 | <0.01 | <0.01 | NS | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | NS | <0.01 | NS | NS | <0.01 | NS | <0.01 | |||||||||||||
| Podocytes per tuft (n) | ||||||||||||||||||||||
| mean | 120 | 130 | 1.1 | 122 | 1.0 | 118 | 125 | 1.1 | 93 | 0.8 | 103 | 137 | 1.3 | 53 | 0.5 | |||||||
| SD | 9 | 11 | 9 | 13 | 17 | 55 | 11 | 28 | 26 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | NS | NS | NS | NS | NS | NS | <0.01 | <0.01 | <0.01 | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | NS | 0.04 | NS | NS | 0.03 | NS | <0.01 | |||||||||||||
| GV/P (μm3) | ||||||||||||||||||||||
| mean | 3907 | 8070 | 2.2 | 11379 | 2.9 | 3939 | 8391 | 2.1 | 52,853 | 16.0 | 3,225 | 9,621 | 3.0 | 62,044 | 19.2 | |||||||
| SD | 663 | 1060 | 1215 | 555 | 1106 | 64,753 | 428 | 2,744 | 34,002 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | <0.01 | <0.01 | <0.01 | <0.01 | 0.01 | 0.01 | NS | <0.01 | <0.01 | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | NS | 0.01 | NS | NS | NS | NS | 0.01 | |||||||||||||
| Mean podocyte volume (μm3) | ||||||||||||||||||||||
| mean | 1833 | 3229 | 1.8 | 4059 | 2.2 | 1734 | 3440 | 2.0 | 4176 | 2.4 | 1133 | 3422 | 3.0 | 2945 | 2.6 | |||||||
| SD | 403 | 592 | 534 | 332 | 377 | 1552 | 116 | 660 | 1217 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | NS | <0.01 | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | NS | <0.01 | NS | NS | 0.01 | NS | NS | |||||||||||||
| GLEPP1-negative tuft volume (μm3) | ||||||||||||||||||||||
| mean | 247,264 | 624,863 | 2.3 | 899,269 | 3.6 | 261,754 | 622,971 | 2.4 | 1,821,775 | 7.0 | 215,214 | 816,892 | 3.8 | 2,530,099 | 12.2 | |||||||
| SD | 42,767 | 43,760 | 152,524 | 67,173 | 176,023 | 897,848 | 35,969 | 195,808 | 363,279 | |||||||||||||
| P value within WT, heterozygous, or homozygous group | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | <0.01 | |||||||||||||
| P value between WT, heterozygous, and homozygous groups | NS | NS | 0.04 | NS | NS | NS | NS | 0.03 | <0.01 | |||||||||||||
Male rats at 100 g either nephrectomy or remained intact. The kidneys obtained by nephrectomy at 100 g were used as a baseline. After 14 weeks on an ad libitum diet, rats were euthanized (with the exception of the homozygous nephrectomy group, which reached ESRD and were euthanized at 9 weeks after nephrectomy). Morphometric variables were measured as shown in the table. The P values for each data set in the upper line compare values within each group. P values are shown in three columns. The left column compares 100-g rats to the intact rat 14 weeks later. The middle column compares the intact rat at 14 weeks to the nephrectomized rat at 9 weeks. The right column compares the nephrectomized rat at 9 weeks to the 100-g rat at 0 weeks. The P values shown for each data set in the lower line compare data for the same variables between wild-type, heterozygous, and homozygous groups. The P values in the wild-type column show values comparing wild-type to heterozygous rats. The data in the middle columns compare heterozygous rats to homozygous rats. The values in the homozygous columns compare homozygous rats to wild-type rats. NX, nephrectomy; WT, wild-type; GV/P, glomerular volume per podocyte.