Figure 2.

Inhibition of LPS–LBP interaction and LPS-induced TNF-α production by structurally different cationic peptides. (A) Biotinylated LPS (45 ng/ml) was added to wells with immobilized LBP in the presence or absence of the indicated cationic peptides (10 μg/ml). The peptides were added to the wells at the same time as the LPS, and residual LPS binding was assessed by ELISA. (B) RAW 264.7 cells were incubated with E. coli O55:B5 LPS (100 ng/ml) in the presence or absence of the indicated peptides (20 μg/ml) for 6 h. TNF-α released into the culture supernatant was measured by ELISA. Data are from ref. 39.