FIG. 4.

Methazolamide affected the glycolytic pathway in FAO hepatoma cells. ECAR as an indicator of glycolytic rate was measured in FAO cells treated with vehicle (0.33% DMSO), MTZ (100 μmol/L), metformin (200 μmol/L metformin [MET]), or MTZ/metformin (cotreatment of 100 μmol/L MTZ and 200 μmol/L metformin) for 16 h. ECAR was determined under basal conditions (2.5 mmol/L glucose alone). Data are presented as the means ± SE of 2 independent experiments whereby each treatment was performed in quadruplicate wells. *P ≤ 0.03 vs. vehicle-treated cells; †P ≤ 0.01 vs. metformin-treated cells.