FIG. 5.

Captopril inhibits gelatinase activity and macrophage migration. A: Islet-transplanted liver isolated from mice injected with captopril was used for gel-gelatin zymography. Gelatinase activity was not present in control liver (lane 1), although it was increased 4 h after islet transplantation (lane 2) and was inhibited by captopril injection (lane 3). M, marker. B: In situ gelatin zymography (green) was performed without (a) or with (b) addition of captopril in islet-transplanted liver sections. Captopril inhibited MMP activity (green) in islet (red) surroundings (b). The nucleus was stained with DAPI. C: Murine peritoneal macrophages were allowed to migrate with or without MMP inhibitors. Numbers are expressed as the percentage of macrophages that migrated toward islet-conditioned medium without inhibitor. Macrophage migration was significantly inhibited by captopril in tumor necrosis factor-α (TNF-α) (**P < 0.001, n = 3) and interleukin-1-β (IL1-β)-treated cell-conditioned medium (**P < 0.001, n = 3). The effect also was significant when macrophages were treated with GM6001 (*P < 0.05, *P < 0.05, n = 3). (A high-quality digital representation of this figure is available in the online issue.)