FIG. 4.

Immunostaining for MCP-1 in nondiabetic control (C) and diabetic (D) apoE KO and RAGE apoE double-KO mice, with and without treatment. ApoE KO (A); RAGE/apoE KO (B); diabetic apoE KO (C); diabetic RAGE apoE KO (D); diabetic RAGE apoE KO + alagebrium (A) 1 mg/kg/day (E); diabetic RAGE apoE KO + quinapril (Q) 30 mg/kg/day (F). Renal cortical MCP-1 expression by RT-PCR (G) and ELISA (H) for n = 6–8 per group. Scale bar = 20 μm. *P < 0.05 vs. apoE KO. §P < 0.05 vs. diabetic apoE KO. ¶P < 0.05 vs. RAGE apoE KO. #P < 0.05 diabetic RAGE apoE KO vs. diabetic RAGE apoE KO + quinapril. †P < 0.05 diabetic RAGE apoE KO vs. diabetic RAGE apoE KO+ alagebrium. (A high-quality digital representation of this figure is available in the online issue.)