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. 2012 Jul 23;7(7):e41542. doi: 10.1371/journal.pone.0041542

Figure 4. PTHrP NLS and C terminus deficiency increases neural cell apoptosis and up-regulate expression levels of CDKIs in the hippocampus.

Figure 4

(A) Representative micrographs of sections from the hippocampus of brains from E18.5, P1, P7 and P14 WT and KI mice stained for apoptotic cells using the TUNEL technique (brown, magnification, ×400). (B) The percentage of apoptotic cells in hippocampus was quantified by computer-assisted image analysis. (C) Western blot analysis of hippocampus protein extracts for N-terminal PTHrP (N-PTHrP), Bmi1, p16, p21, p27 and p53. β-tubulin was used as loading control. (D) N-PTHrP, Bmi1, p16, p21, p27 and p53 protein levels relative to β-tubulin protein level were assessed by densitometric analysis and expressed relative to levels of WT mice. Each value is the mean±SEM of determinations in 5 mice of each group. ***, P<0.001 in Pthrp KI mice relative to wild-type littermates. ###, P<0.001 at the time point relative to the prior observed time point in WT mice. ▴▴▴, P<0.001 at the time point relative to the prior observed time point in Pthrp KI mice.