Figure 4. IL-1β maturation and secretion in lungs of mice infected with different P. aeruginosa T3SS mutants.

C57BL/6 mice were infected intratracheally with 1.1⁶ cfu of WT P. aeruginosa or ΔSTY or ΔSTY/ΔPopB mutants, and after 4.5 h, BALF was isolated and analyzed by SDS-PAGE and western blotting for the presence of mature IL-1β. The corresponding total lung extracts were also analyzed by western blotting for the presence of pro IL-1β. Results are representative of six different mice per experimental condition.