Figure 3. Impact of THs on macrophage cytokine production.

(A) Peritoneal macrophages were collected from mice treated with T4 or vehicle as described in Figure 1. (B) RAW264.7 cells and (C) PMA-differentiated THP-1 cells were treated with 100 nM T3 or 1 µM T4 for 24 h, cells treated with vehicle NaOH were set as control. Cells were infected with FAM20 at a MOI of 200 for 24 h and levels of IL-6 and TNFα in the supernatant were quantified by ELISA. The experiments were performed in triplicate and data are presented as means ±SD.