Table 3. Median fluorescence intensity (MFI) of CD86 and MHC class II on CD19⁺ or CD11c⁺ cells in draining lymph nodes (dLNs) and spleen post Alum treatment.

		CD86 (×103)		MHC class II (×103)
		24 h	48 h	24 h	48 h
CD19 dLNs	Naïve	0.8±0.1	0.8±0.1	40±8	40±8
	Alum	0.8±0.1	0.7±0.02	44±4	37±3
CD11c dLNs	Naïve	6.2±6.0	6.2±6.0	34±17	34±17
	Alum	9.2±2.5	18±9.0*	42±13	54±10
CD19 Spleen	Naïve	1.8±0.1	1.8±0.1	24±4	24±4
	Alum	1.9±0.1	1.9±0.05	22±2	23±2
CD11c Spleen	Naïve	2.4±0.2	2.4±0.2	37±0.4	37±0.4
	Alum	2.4±0.1	2.5±0.1	34±2	37±2

Mice were injected subcutaneously at the base of the tail and the draining lymph nodes and spleen was harvested and analyzed as described in Materials and Methods. Data is shown as mean ± SD (n = 3–6 mice). Significant differences from naïve mice using Mann-Whitney test are outlined with *, p<0.05; **, p<0.01; ***, p<0.001.