Skip to main content
. 2012 Jul 23;7(7):e41787. doi: 10.1371/journal.pone.0041787

Figure 5. Clonal stability with respect to antibody expression and amplified genes.

Figure 5

Two high-producer clones with amplified genes at a chromosomal line HSR (A; CN19-10/1A3) or a ladder HSR (B; CN19-4/1F3) were subjected to prolonged culture. The antibody expression in the presence or absence of butyrate (A, B), the copy number of the Ig-light chain (MycL) and the IR sequence per cell (A’, B’), and the amplified structure were examined by ELISA, real-time PCR and FISH, respectively, at the days after the plasmid transfection that were noted in the graph.