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. 2012 Jul 23;7(7):e41787. doi: 10.1371/journal.pone.0041787

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Araki et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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The high producer cell clones (A, CN19-10/1A3; B, CN19-4/1F3) were adapted to suspension serum-free culture as described in the Materials and Methods section. The cell density and the antibody concentration in the medium are shown in the Graphs. The arrows indicate the time points at which the entire medium was changed.