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. 2012 Jul 23;7(7):e41891. doi: 10.1371/journal.pone.0041891

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Narita et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A–C. H1299 cells were transfected with various combinations of expression vectors for FLAG-MM-1α, HA-wild-type Rabring7, HA-C229S Rabring7 and FLAG- or EGFP-c-Myc by Lipofectamine 2000. Forty-eight hrs after transfection, the cells were fixed with 4% paraformaldehyde and reacted with anti-FLAG, anti-HA and lamin B antibodies. The cells were then reacted with FITC-conjugated anti-rabbit and rhodamine-conjugated anti-mouse antibodies. A: transfection with FLAG-MM-1α, with FLAG-c-Myc or with HA-wild-type Rabring7 or HA-C229S Rabring7 alone. B: co-transfection with FLAG-MM-1α or FLAG-c-Myc and HA-Rabring7 or HA-C229S Rabring7. C: co-transfection with FLAG-MM-1α, A-wild-type Rabring7 or HA-C229S Rabring7 and EGFP-c-Myc.