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. 2012 Jul 23;7(7):e41891. doi: 10.1371/journal.pone.0041891

Figure 7. Effect of Rabring7 on stability of c-Myc.

Figure 7

A. HEK293T cells were co-transfected with a constant amount of FLAG-MM-1α and with various doses of T7-Rabring7, and proteins in cells were analyzed by Western blotting with anti-T7, anti-FLAG and anti-actin antibodies. B. HEK293T cells were co-transfected with constant amounts of FLAG-MM-1α and FLAG-c-Myc together with various doses of T7-Rabring7, and proteins in cells were analyzed by Western blotting with anti-T7, anti-FLAG and anti-actin antibodies. C. Expression levels of MM-1α, Rabring7 and actin in vector- and MM-1-expressing H1299 cells were analyzed by Western blotting with anti-MM-1, anti-Rabring7 and anti-actin antibodies. D. Vector- and MM-1-expressing H1299 cells were transfected with a constant amount of FLAG-c-Myc and with various amounts of T7-Rabring7. At 48 hrs after transfection, protein in the cells were analyzed by Western blotting with anti-T7, anti-FLAG and anti-actin antibodies. E. The intensity of bands corresponding to FLAG-c-Myc and actin in Fig. 6D was quantified, and relative intensity of FLAG-c-Myc to that of actin is shown. Values are means ± S.D. n = 3 experiments. Significance: **p<0.01 and ***p<0.001.