| F1A(BamH I) |
AGGGATCCGGCAGTTAAGCAGAAAGT
|
fis mutant construction |
| F1B(Sal I) |
CTGTCGACGTTACCTGATCCTGAGAGTT
|
|
| F2A(Sal I) |
TATGTCGACTGCTGCTCTGATGATGG
|
|
| F2B(BamH I) |
TCAGGATCCCACCATACCGTCGAAAT
|
|
| P-fis-A(Nco I) |
TACCATGGATACGCTATTGAGGACGC
|
Complementary expressionof fis in Δfis
|
| P-fis-B(Sal I) |
CTGTCGACTTAGTTCATGCCGTATT
|
|
| Pa(23173) |
TAACAGATAGCCACACACAGT
|
Confirmation of the presenceor absence of pBSSB1 by PCR |
| Pb(23750) |
TCAAGGAAGACTGAGATTTGT
|
|
| P-Kana-A |
AATTGATAAAGGAAAGTGGTTCCGTTATAAAAATGGCTTATTCGATATAAGGTCTGACGCTCAGTGGA
|
Insertion of kanamycin cassettewithin pBSSB1 |
| P-Kana-B |
TAGTGGCTCAAAGAGTATTAGAAATTGACAGAGAAAAGAAAGCAGAATGATTTCGGCCTATTGGTTAA
|
|
| ORF1-A |
GAGAAGATGCCCGTAA
|
Confirmation of the insertionof kanamycin cassette withinpBSSB1 by PCR |
| Kana-B |
ATGGCTCATAACACCC
|
|
