Figure 2. L-Arginine transport kinetics.
L-Arginine transport kinetics (3 μCi/mL L-[3H] arginine, 1 minute, 37°C) in HUVECs cultured (8 hours) without (–NBTI; a, c) or with (+NBTI; b, d) 10 μmol/L nitrobenzylthioinosine (NBTI). (a, b) Saturable L-arginine transport in absence (control) or presence of 1 nmol/L insulin and/or ZM-241385 (10 nmol/L) or CGS-21680 (30 nmol/L) as indicated. (c, d) Eadie-Hofstee plot of the L-arginine transport data in (a) and (b), respectively. Values are mean ± SEM (n = 6–18).