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. 2012 Jul 23;7(7):e41705. doi: 10.1371/journal.pone.0041705

Figure 4. hCAT-2A/B protein abundance in response to insulin and adenosine receptor agonists and antagonists.

Figure 4

(a) Western blots (representative of other 6 experiments) for hCAT-2A/B in HUVECs incubated (8 hours) without (–NBTI) or with (+NBTI) 10 μmol/L nitrobenzylthioinosine (NBTI), in the absence (–) or presence (+) of 1 nmol/L insulin, CGS-21680 (30 nmol/L) and/or ZM-241385 (10 nmol/L) (ß-actin is internal control). Lower panel: hCAT-2A/B/ß-actin ratio densitometries from data in absence (white bars) or presence (black bars) of NBTI normalized to 1 in cells in the absence of NBTI, insulin, CGS-21680 or ZM-241385 (control). Values are mean ± SEM (n = 6).