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. 2012 Jul 23;7(7):e41705. doi: 10.1371/journal.pone.0041705

Figure 6. Involvement of adenosine receptors on insulin effect in human umbilical vein rings reactivity.

Figure 6

(a) Endothelium-intact (circles) or endothelium-denuded (squares) human umbilical vein rings were exposed (8 hours) to increasing concentrations of insulin in absence (○,□) or presence (•,▪) of ZM-241385 (10 nmol/L). (b) Endothelium-intact human umbilical vein rings incubated in absence (–, control) or presence (+) of 10 μmol/L nitrobenzylthioinosine (NBTI) and/or ZM-241384 as in (a). Reactivity was measured in vessel rings co-incubated without (white bars) or with (black bars) 1 nmol/L insulin. Relative responses are given as a percentage fraction of the initial vessel response to KCl (see Methods). (c) Umbilical vein rings with (+ Endothelium) or without (– Endothelium) were exposed for 2 minutes or 8 hours 30 nmol/L CGS-21680 (white bars) in the absence or presence of 10 nmol/L ZM-241385 (black bars) or 10 nmol/L DPCPX (grey bars). Relative responses are as in (a). In (a), *P<0.05 versus corresponding values in endothelium-intact vessels. In (b), *P<0.05 versus control. In (c), *P<0.05 versus all other values except in DPCPX for 2 minutes in endothelium intact vessels. Values are mean ± SEM (n = 7–9).