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. 2012 Jul 23;7(7):e40931. doi: 10.1371/journal.pone.0040931

Figure 2. Rvs proteins are needed for the effect of AtxA on endocytosis.

Figure 2

A Average Sla1-GFP patch lifetimes for different rvs deletion strains and yap1802Δ deletion strain expressing AtxA from a single copy integrated in the genome, and corresponding control strains. B Localization of Sla1-GFP in rvs161Δ, rvs167Δ, the rvs161Δ rvs167Δ double deletion strain and yap1802Δ. C Effect of additional expression of either RVS161 or RVS167 on average Sla1-GFP patch lifetimes for WT and rvs deletion strains expressing AtxA from a single copy integrated in the genome and corresponding control strains. D Average Sla1-GFP patch lifetimes for the wild-type and bmh1Δ and bmh2Δ deletion strains expressing AtxA from plasmid, and corresponding control strains. E Average lifetimes of Rvs161-GFP (left) and Rvs167-GFP (right) patches for the wild-type (ctrl) and AtxA-expressing cells. F Localization of Rvs161-GFP and Rvs167-GFP in AtxA-expressing and control cells. The bars on graphs mark the standard deviation. To determine the average patch lifetimes at least 100 patches from several cells were analyzed. Sla1-GFP movies were taken with a 1 frame/second and Rvs161-GFP and Rvs167-GFP movies with a 4 frames/second interval. Scale bar on the micrographs is 4 µm. AtxA-expressing strains were compared to the corresponding control strains, and p-values were calculated using the t-test at a 95% confidence interval. Statistically significant differences are denoted with a star (*).