Figure 5. Several neurotoxic sPLA₂s bind to yeast 14-3-3 proteins.

All tested neurotoxic sPLA₂s as well as AtnL bind to both Bmh1 and Bmh2. No binding was detected for the nontoxic AtnI₂ and ppPLA₂. Responses in response units (RU) were corrected for surface density of Bmh1 and Bmh2 and molecular weight of Bmh1, Bmh2 and sPLA₂. The bars represent standard deviations of at least three independent measurements. AtxA – ammodytoxin A, AtxB – ammodytoxin B, AtxC – ammodytoxin C, bvPLA₂– bee venom PLA₂, bButx – β-bungarotoxin, AtnL – ammodytin L, Tpx – taipoxin, AtnI₂– ammodytin I₂, ppPLA₂– porcine pancreatic PLA₂.