Table 1. Binding of yeast 14-3-3 proteins to phospholipid vesicles.
5 µM Bmh1 | 5 µM Bmh2 | 5 µM Bmh1+2 | ||||
Av. | s.d. | Av. | s.d. | Av. | s.d. | |
50 nm | 69.4 | 24.6 | 44.1 | 5.5 | 48.5 | 2.0 |
100 nm | 66.7 | 25.1 | 47.3 | 16.4 | 47.2 | 0.9 |
200 nm | 70.2 | 22.0 | 46.7 | 7.1 | 54.8 | 0.8 |
Surface plasmon resonance was used to analyze binding of 14-3-3 proteins to phospholipid vesicles. The response after 180 seconds of dissociation phase is shown. Av. – average response in response units (RU), s.d. – standard deviation in RU of at least three independent measurements.