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. 2012 Jul 23;7(7):e41092. doi: 10.1371/journal.pone.0041092

Figure 3. Activities of the ISRE-promoter luciferase reporter genes.

Figure 3

(A) IFN-α-responsive ISRE-luc promoter activity. The cells were treated with recombinant IFN-α (100∼5000 U) for 8 h at 24 h after transfection. The gray columns represent the RLA levels in cells transfected with empty plasmid, whereas the black columns represent the RLA levels in cells transfected with ISKNV-vSOCSmyc plasmid. RLA level in cells transfected with TA-luc reporter gene instead of the ISRE-luc reporter gene was used as a negative control. RLA levels of cells transfected with empty plasmid without stimulation were arbitrarily set as 1. (B) Activities of reporter genes in cells transfected with increasing amounts of ISKNV-vSOCSmyc plasmid. Cells were transfected with different amounts of ISKNV-vSOCSmyc plasmid (1∼200 ng), treated with IFN-α (5000 U) for 8 h, and then ISRE-luc activity was analyzed. RLA levels in cells transfected with empty plasmid after IFN-α treatment were arbitrarily set as 1. Error bars represent the mean ± S.D. (n = 3). **P<0.01.