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. 2012 Jul 23;7(7):e40873. doi: 10.1371/journal.pone.0040873

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Chu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Amino acid sequences derived from the insert DNA are capitalized, lipobox is underlined, the cleavage site is indicated by an arrow, and amino acid sequences of mature proteins are bolded. (B) Purified recombinant lipidated (L-Ag473) and non-lipidated (NL-Ag473) Ag473 in PBS, were run on a 15% SDS-polyacrylamide gels and subjected to silver staining or Western blotting (right panel).