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. 2012 Jul 23;7(7):e40873. doi: 10.1371/journal.pone.0040873

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Chu et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Antibody titers (A) and the IgG isotype profiles (B) of the Ag473-immune sera determined by ELISA. (A) AP- anti-mouse Ig (μγα) was used as the secondary antibody; (B) anti-NL-Ag473 was 100X diluted while others were 1000X. The shown readings were obtained by subtracting the readings of preimmune sera from that of immune sera.