Figure 3. L-Ag473, but not NL-Ag473 activates BMDCs and macrophages.

(A) BMDCs were treated with L-Ag473 or NL-Ag473 (100 ng/ml) (black line) for 6 hours and the intracellular TNF-α of CD11c⁺ cells were determined by flow cytometry. Dotted and grey lines represent the isotype-matched control Ab and untreated cell, respectively. (B, C) Dose-dependent response of BMDC (B) and macrophages (C). ^NS p>0.05; *p<0.05; **p<0.01 (Student’s t-test), comparing Ag473-treated to non-treated cells. All data are representative of two to four independent experiments.