Figure 4. The biological activity of L-Ag473 is retained after pretreatment with PMB but abolished by heat or proteinase K treatment.

BMDCs were treated with L-Ag473 (100 ng/ml) or LPS (20 ng/ml) for 6 hours and the intracellular TNF-α of CD11c⁺ cells were determined by flow cytometry. Dotted and grey lines represent the isotype-matched control Ab and untreated cell, respectively. PNaseK, proteinase K.