Figure 5. L-Ag473 induces cytokine and chemokine production by BMDCs.

BMDCs were incubated with L-Ag473 for 24 hours (or 6 hours for TNF-α and RANTES). Supernatants were collected and (A) TNF-α, IL-6, and IL-12; (B) MCP-1, MIP-1, and RANTES were determined by ELISA. Data are shown as mean ± SD from triplicate DC cultures; ^NS p>0.05; *p<0.05; **p<0.01 (Student’s t-test), comparing Ag473-treated to non-treated cells. All data are representative of three independent experiments.